K T C C 2005

4th International Symposium of Kyoto T Cell Conference

President; Sonoko Habu
Program Committee; Shigeo Koyasu, Nagahiro Minato, Toshinori Nakayama, Takashi Saito, Shimon Sakaguchi, Yousuke Takahama.

The '4th International Symposium of Kyoto T Cell Conference' was held in conjunction with '2005 Workshop of Thymus Organogenesis' and 'Symposium on Immune Tolerance and Regulation'.


Pictures from the meetings

Additional large picture files

Contribution of additional pictures is highly welcome. Please send the digital files to Y. Takahama.


at Shiran Kaikan, Kyoto University, Kyoto, Japan

April 6

15:00 Registration

18:00 Welcome remarks
18:05 - 19:30 Keynote lecture, Thomas Boehm (Max-Planck-Institute of Immunobiology) Genetic dissection of thymus development: Uncharted aspects of its phylogeny and ontogeny

20:00 Reception at Hotel Fujita

April 7


The organogenesis of the thymus, Chair: Graham Anderson

9:00 - 9:30 Georg Hollander (University of Basel) On the molecular mechanisms of early thymus development
9:30 - 10:00 Andrew Farr (University of Washington) Extrathoracic thymic tissue in the mouse - isnt one thymus enough?
10:00 - 10:15 Norimasa Iwanami (University of Tokushima) Medaka mutations affecting thymus organogenesis
10:15 - 10:30 Hisahiro Yoshida (RCAI RIKEN) Screen of immune disease model mice from ENU-induced mutant pool


Thymic epithelial cells, Chair: Georg Hollnder

11:00 - 11:30 Clare Blackburn (University of Edinburgh) Thymic epithelial progenitor cells
11:30 - 12:00 Hans-Reimer Rodewald (University of Ulm) Insights into the role of the DiGeorge syndrome gene Tbx1 for thymus development from nude mouse blastocyst complementation
12:00 - 12:15 Shigeru Koshiba (Sapporo Medical University School of Medicine) p53-related transcription factors modulate intercellular connections of thymic medullary stromal cells through tight junctions
12:15 - 12:30 Ann Chidgey (Monash University) Reversal of thymic involution by sex steroid ablation: a molecular analysis

Epithelial-mesenchymal interactions for thymus development, Chair: Nancy Manley

14:30 - 15:00 Graham Anderson (University of Birmingham) Role of mesenchyme in thymus development and function
15:00 - 15:30 Ellen Richie (University of Texas) Thymus organogenesis in Pax3 sp/sp embryos
15:30 - 15:45 Hidetoshi Yamazaki (Tottori University) Neural crest-derived cells in the murine developing thymus and their potential for differentiation
15:45 - 16:00 Julie Gordon (University of Georgia) The role of BMP signaling in patterning the common thymus/parathyroid primordium


Lympho-stromal interactions for thymus function, Chair: Bruno Kyewski

16:30 - 17:00 Willem van Ewijk (Leiden University) The Notch signalling pathway is involved in thymic crosstalk
17:00 - 17:30 Nancy Manley (University of Georgia) The Foxn1D/D thymic microenvironment supports T cell development from non-canonical progenitors
17:30 - 18:00 Manami Itoi (Meiji University of Oriental Medicine) The thymic perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma
18:00 - 18:15 Taishin Akiyama (University of Tokyo) TRAF6 in thymic stroma is essential for self-tolerance
18:15 - 18:30 Daniel Gray (Monash University) The relative contribution of thymic stromal cell subsets to negative selection
18:30 - 18:45 Mitsuru Matsumoto (University of Tokushima) Essential role of I_B kinase _lpha in thymic organogenesis required for the establishment of self-tolerance
18:45 - 19:00 William Jenkinson (University of Birmingham) Investigating the role of thymic mesenchyme in the regulation of thymocyte development


Symposium on Immune Tolerance and Regulation, Chair: Kayo Inaba

9:00 - 9:30 Shimon Sakaguchi (Kyoto University) Control of immune responses by naturally occurring Foxp3-expressing CD25+CD4+ regulatory T cells
9:30 - 10:00 Ethan Shevach (NIH) Naturally occurring CD4+CD25+ regulatory T cells (nTR) function as a rapid response force to control effector T cell activation
10:00 - 10:30 Alexander Rudensky (University of Washington) Development of Foxp3-expressing Regulatory T cells


Chair: Hitoshi Kikutani

11:00 - 11:30 Naoko Nakano (Tokyo University of Science) Homeostatic balance between nave and CD25+ regulatory T cells recognizing an altered peptide ligand expressed as a self-antigen
11:30 - 12:00 Miyuki Azuma (Tokyo Medial and Dental University) Dual functions of B7-H1 and B7-DC in immune responses
12:00 - 12:30 Lieping Chen (Johns Hopkins University School of Medicine) New co-signaling pathways in immune regulation

Chair: Miyuki Azuma

14:30 -15:00 Hitoshi Kikutani (Osaka University) Semaphorins in the Immune System
15:00 -15:30 Vijay Kuchroo (Harvard Medical School) TIM family of genes: role in T cell differentiation, autoimmunity and tolerance
15:30 -16:00 Kayo Inaba (Kyoto University) Plasmacytoid dendritic cells in human and mouse


Chair: Shimon Sakaguchi

16:30 - 17:00 Pamela Ohashi (University of Toronto) Examining tolerance and immune surveillance
17:00 - 17:30 Sachiko Hirose (Juntendo Univeristy) Genetic aspect of aberrant activation of self-reactive B cells in systemic autoimmune disease
17:30 - 18:00 Kazuhiko Yamamoto (University of Tokyo) TCR gene transfer for controlling autoimmune diseases and immune-mediated disorders TCR gene transfer for controlling autoimmune diseases and immune-mediated disorders

April 8

Dynamics of developing thymocytes, Chair: Howard Petrie

9:00 - 9:30 Howard Petrie (Scripps Florida) T lineage commitment: extrathymic or intrathymic?
9:30 - 10:00 Conrad Bleul (Max-Planck-Institute for Immunobiology) A recent thymic immigrant maps to the branching point of the T versus B lineage decision
10:00 - 10:30 Yousuke Takahama (University of Tokushima) Cortex-medulla migration of thymocytes and central tolerance
10:30 - 11:00 Nikolaus Trede (University of Utah) In-vivo imaging in zebrafish &endash; a novel tool to tackle the genetics of autoimmunity


T lymphopoiesis, Chair: Juan Carlos Ziga-Pflucker

11:30 - 12:00 Irving Goldschneider (University of Connecticut) Prothymocyte development is defective in IL-7Ra-/-, but not IL-7-/-, mice
12:00 - 12:15 Harumi Suzuki (Yamaguchi University) Effect of LIF on T-poiesis from multipoteintial Pax5 deficient pro-B cells
12:15 - 12:30 Shinya Ogawa (Kyoto University) Regulation of IL-7 receptor expression during T cell development
12:30 - 12:45 Shinji Fujimoto (Kyoto University) Forced Id2 expression generates NK cells from fully specified T cell precursors in murine fetal thymus
12:45 - 13:00 Yasutoshi Agata (Kyoto University) E2A proteins induce chromatin accessibility for V(D)J recombination through the alteration of histone modification

Early thymocyte development, Chair: Eric Jenkinson

15:00 - 15:30 Robson MacDonald (Ludwig Institute for Cancer Research) Role of Notch and pre-TCR signaling in T cell development and lineage commitment
15:30 - 16:00 Juan Carlos Zuniga-Pflucker (University of Toronto) The importance of Notch signals at the b-selectioncheckpoint
16:00 - 16:15 Masaki Miyazaki (Hiroshima University) Stability of Polycomb group protein influences intrathymic T cell development during DN to DP transition
16:15 - 16:30 Toshio Watanabe (Tohoku University) Forced expression of either one of two Runx1 isoforms exert differential effects on the thymocyte development


Lineage determination to T cells, Chair: Hiroshi Kawamoto

17:00 - 17:30 Eric Jenkinson (University of Birmingham) T/B lineage choice occurs prior to intrathymic Notch signaling
17:30 - 17:45 Katsuto Hozumi (Tokai University) Pre-thymic Notch signaling is necessary for GATA3 to work in the earliest stage of T cell development
17:45 - 18:00 Arivazhagan Sambandam (University of Pennsylvania) Notch signaling controls the generation and differentiation of early T lineage progenitors
18:00 - 18:30 Hiroshi Kawamoto (RCAI RIKEN) Macrophage potential retained by early thymic T cell progenitors

18:30 -19:30 Posters with drink

P1 Michiyuki Kasai (NIID) A possible involvement of autophagy in presentation of cytoplasmic antigens via MHC class II molecules
P2 Junko Chiba (Nippon Veterinary and Animal Science University) Abnormal postnatal development of hypoplastic thymus in a new dwarf mutant rat strain PET
P3 Shizuko Kakinuma (NIRS) Cytokine production by thymic adherent cells during radiation-induced thymic lymphomagenesis
P4 Yi Shang (NIRS) Expression of cytokine receptors in radiation-induced mice thymic lymphoma
P5 Cunlan Liu (University of Tokushima) Differential roles of CCR7 and DOCK2 in cortex-medulla migration of positively selected thymocytes
P6 Hirofumi Shibata (Kyoto University) Regulation of IL-7 receptor expression by T cell receptor signal
P7 Atsushi Irie (Kumamoto University) PKD2, the major isoform of PKD family kinases expressed in the T cells and thymocytes, is involved in TCR-mediated T cell activation
P8 Jun Tsukada (RCAI RIKEN) Notch signals have a distinct role for nave and memory CD4 T cells
P9 Hirotake Tsukamoto (Kumamoto University) TCR ligand avidity determines the mode of B-RAF/MEK/ERK activation leading to proliferative response in human CD4+ T cells
P10 Ryoji Yagi (RCAI RIKEN) CNS-2 is key element for IL-4 production in initial IL-4 producer cells
P11 Shinya Hirata (Kumamoto University) Prevention of experimental autoimmune encephalomyelitis via induction of T cells with regulatory function by transfer of ES-DC expressing MOG peptide along with TRAIL

19:30 Getting together

April 9

Transcriptional regulation of T cell differentiation, Chair: Ellen Rothenberg

9:00 - 9:30 Ellen Rothenberg (California Institute of Technology) Transcription factor interplay in T lineage commitment
9:30 - 10:00 Takehito Sato (Tokai University School of Medicine) Dual functions of Runx proteins for re-activating CD8 and silencing CD4 at the commitment process into CD8 thymocytes
10:00 - 10:15 Kazuyoshi Kohu (Tohoku University) Over-expression of the Runx3 transcription factor increases the proportion of mature thymocytes of the CD8 single positive lineage
10:15 - 10:30 Ichiro Taniuchi (RCAI RIKEN) Roles of Runx transcriptional factor complex during lymphocyte development


T cell selection, Chair: Sonoko Habu

11:15 - 11:45 Sho Yamasaki (RCAI RIKEN) Molecular mechanism of pre-TCR-mediated signal transduction
11:45 - 12:15 Osami Kanagawa (RCAI RIKEN) The ability of individual thymocytes to generate multiple VJ alpha rearrangements is critical for normal T cell development
12:15 - 12:45 Alfred Singer (National Cancer Institute) Cytokine regulation of coreceptor expression: Implications for T cell selection and reactivity
12:45 - 13:00 Keiko Udaka (Kochi Medical School) Raft-associated presentation of MHC class II molecules guides thymocytes to the CD4 lineage

Signals regulating T cell development and responses, Chair: Takashi Saito

15:00 - 15:30 Michael Dustin (New York University) Patterns of tyrosine phosphorylation in the immunological synapse
15:30 - 16:00 Satoshi Matsuda (Keio University) Spry1 functions as a negative-feedback loop in the TCR signaling pathway
16:00 - 16:30 Rose Zamoyska (National Institute for Medical Research) Contrasting roles for p56lckand p59fyn in regulating nave T cell responses
16:30 - 16:45 Yoshinori Fukui (Kyushu University) DOCK2, a regulator of the actin cytoskeleton in lymphocytes, is anovel molecular target for controlling allo-graft rejection and autoimmune diseases
16:45 - 17:00 Hidehiro Kishimoto (Tokyo University of Science) Distinct function of NF-kB2-relB activation between semi-mature thymocytes and mature T cells


T cell recognition and responses, Chair: Shigeo Koyasu

17:30 - 18:00 Joost van Meerwijk (INSERM) Molecular signature of recent thymic selection events on effector and regulatory CD4+ T lymphocytes
18:00 - 18:30 Leonore Herzenberg (Stanford University) Culturing lymphocytes at atmospheric oxygen levels impacts T cell function
18:30 - 18:40 Kazuko Shibuya (University of Tsukuba) Requirements of the serine at residue 329 for lipid rafts recruitment of DNAM-1 (CD226)
18:40 - 18:50 Shuhei Tomita (University of Tokushima) Hypoxia inducible factor-1a regulates in vivo T cell responses
18:50 - 19:00 Takamasa Ueno (Kumamoto University) Functionally impaired HIV-specific CD8 T cells show high-affinity TCR-ligand interactions
19:00 - 19:10 Sachiko Kondo (Toyama Medical and Pharmaceutical University) Detection of antigen-specific lymphocytes with microwell-array system
19:10 - 19:20 Yoshimasa Tanaka (Kyoto University) Analysis of mechanism for nonpeptide antigen recognition by human gd T cells
19:20 - 19:30 Satoru Senju (Kumamoto University) Therapeutic control of immune responses by genetically modified ES cell-derived dendritic cells

April 10

Signals regulating helper T-cell differentiation, Chair: Toshinori Nakayama

8:30 - 9:00 Mark Bix (University of Washington) Chromosome specific analysis of cis-acting DNA regulatory elements on Il4 locus
9:00 - 9:30 Masakatsu Yamashita (Chiba University) Ras-Erk MAPK cascade, GATA3, and the ubiquitin-proteasome pathway in Th2 differentiation
9:30 - 9:45 Shinsuke Taki (Shinshu University) Homeostatic regulation of the Th1/Th2 balance by controlling basophil population size
9:45 - 10:00 Motoko Kimura (Chiba University) Regulation of Th2 cell differentiation by murine schnurri-2
10:00 - 10:15 Masato Kubo (RCAI RIKEN) Regulation of initial IL-4 production in memory like Th2 cells by RBP-J/Notch signaling through conserved non-cording sequence (CNS)-2 on IL-4 locus


T cell homeostasis and memory, Chair: Rose Zamoyska

10:45 - 11:15 Janko Nikolich-Zugich (Oregon Health & Science University) T-cell repertoire generation, diversity, maintenance and aging
11:15 - 11:30 Akemi Sakamoto (Chiba University) Bcl6 acts as an amplifier for the generation of central memory CD8 T cells
11:30 - 11:45 Shinya Tanaka (RCAI RIKEN) The physiological role of intrinsic memory-like T cells expressing IL-4 by primary antigen-stimulation
11:45 - 12:00 Hiroyuki Tezuka (Akita University) Impairment of APRIL-dependent IgA class-switch recombination in iNOS-/- mice
12:00 - 12:30 Elizabeth Simpson (Hammersmith Hospital) T cell responses to endogenous epitopes: balancing tolerance and immunity

Sandwich and coffee

Immune regulation and diseases, Chair:Nagahiro Minato

13:15 - 13:45 Shimon Sakaguchi (Kyoto University) Development of autoimmune arthritis due to a mutation of the ZAP-70 gene: T cell selection shift and altered Treg function
13:45 - 14:15 Bruno Kyewski (German Cancer Research Center) Promiscuous gene expression in thymic epithelial cells is regulated at multiple levels
14:15 - 14:45 Richard Boyd (Monash University) Restoration of thymic function and enhancement of haemopoiesis and lymphopoiesis following haemopoietic stem cell transplantation: re-shaping immunity for health
14:45 - 15:00 Wilfred Germeraad (University Hospital Maastricht) Tumour associated antigen mucin-1 is expressed on medullary epithelial cells in the thymus
15:00 - 15:15 Kenichiro Seino (Juntendo University) Immunoregulatory T cells and transplantation tolerance
15:15 - 15:45 Nagahiro Minato (Kyoto University) Chronic leukemia and systemic autoimmunity as hematopoietic stem cell disorders

Closing remarks

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Last update;April 22, 2005