Welcome to the 3rd KTCC meeting.

The KTCC was founded in 1991 to provide an opportunity for researchers in Japan working on T cells and related fields to exchange scientific ideas through intensive discussions. The KTCC puts the highest value on the discussions based on the work and thoughts of each group. Since such an opportunity has hardly been encountered in Japan until recently, the organizers are still looking for a better arrangement of the meeting. Any problem pointed out by participants will be considered for the next meeting.

It is our great pleasure to be able to invite foreign researchers from a variety of countries. However, because there are many difficulties for most Japanese researchers to have a hot discussion in English, we have decided to divide the meeting into Part I (oral presentation and discussion in Japanese) and Part II (symposium in English). To provide an opportunity for all participants to freely communicate with each other, poster sessions have been arranged in the evenings of October 14 and 15, where all oral presentations are displayed as posters in English.


KATSURA Yoshimoto
HIAI Hiroshi
MINATO Nagahiro

Program of the 3rd KTCC meeting

Date: October 14−16, 1993

Site: JT Kyoto Kaikan


Session IA Stem cells(Chairperson: Nakauchi, H.)

1. Matsuzaki, Y. (RIKEN, Tsukuba)

Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation.

2. Uchida, N. (Stanford Univ.)

Heterogeneity of mouse hematopoietic stem cells separated by counter-flow centrifugal elutriation.

3. Ikuta, K. (Tokyo Univ.)

N nucleotide insertion capacity of single hematopoietic stem cells.

4. Hirata, M. (Osaka Univ.)

Establishment and characterization of a hematopoietic stem cell line, HSC-1.

Related posters

39. Wu, L. (The Walter and Eliza Hall Inst.)

Characterisitics of the earliest T-precursor cell in the mouse thymus.

40. Wang, J-F. (Inst. Rad. Med., Beijing)

The production of hematopoietic inhibitory activity from the T lymphocytes stimulated by McAb OKT3 depends on the presence of IL-2 or autologous adherent bone marrow cells and the expression of HLA-DR antigen.


Session IB Committed precursors(Chairperson: Habu, S.)

5. Kasai, M. (NIH, Tokyo)

Molecular analysis of the recombination hotspot associated proteins specifically expressed in immature T and B lymphoid cells.

6. Inui, S. (Tottori Univ.)

Establishment of a monoclonal ab which inhibits the growth of murine pre B cells.

7. Hozumi, K. (Tokai Univ.)

Characterization of pro-T cells in fetal murine thymus.


Session II Lymphocyte differentiation and microenvironment(Chairperson: Uede, T.)

8. Takeuchi, T. (NCNP, Tokyo)

In vitro arrest of immature T cell development by a novel monoclonal antibody, HS9.

9. Mizuochi, T. (NIH and TMIG, Tokyo)

Molecular analysis for the difference between cortical and medullary thymic epithelial cells.

10. Tanaka, Y. (NIMR, UK)

Negative selection ofαβTCR transgenic thymcoytes by conditionally immortalised thymic cortical epithelial cells.

11. Takayama, E. (Kyoto Univ.)

Molecular and cellular analysis of mouse VCAM-1 expression in hematopoietic system.

12. Kishi, H. (Kyushu Univ.)

Cell surface antigen expressed on thymocytes differentiating from CD4- 8- to CD4++.

13. Arase, H. (Hokkaido Univ.)

Cytotoxicity of CD4- CD8- TCRαβpositive thymocytes against thymocyte or tumor cell target.

14. Amagai, T. (Meiji College of Oriental Med., Kyoto)

Characterization of basophilic cells in nude mouse thymus.

Related posters

41. Platenburg, P. P. (Erasmus Univ., The Netherlands)

Inhibition of T-cell differentiation by CD44 mAb.

42. Nanno, M. (Tohoku Univ.)

Analysis of hematopoietic activity of a murine fetal hepatocyte clone via cell contact.


Session IIIA Transcription factors(Chairperson: Tokuhisa, T.)

15. Hu, L. (Chiba Univ.)

A role of AP-1 in proliferation and differentiation of pre-B cells.

16. Fujimoto, S. (Kyoto Univ.)

Analysis of the products derived from alternative splicing of the lymphoid enhancer-binding factor LEF-1 gene.

17. Satake, M. (Tohoku Univ.)

Murine transcription factor, PEBP2α, homologous to the human AML1 gene, is expressed during thymic cell development.

18. Yamamoto, M. (Tohoku Univ.)

Expression of GATA-3 in T lymphocytes and neuroblastoma cells.

Session IIIB Growth mechanism(Chairperson: Nakayama, T.)

19. Hiai, H. (Kyoto Univ.)

Genetic predisposition to pre-B lymphomas in SL/kh mice.

20. Misawa, Y. (NIH, Tokyo)

Stimulation of CD43 receptor breaks cell cycle arrest in a mature B cell line.

21. Utsuyama, M. (TMIG, Tokyo)

Analysis of genes encoding signal transduction proteins in mouse thymus during ontogenesis.

22. Ogata, M. (Osaka Univ.)

cDNA cloning of protein tyrosine phosphatases expressed in lymphoid tissues.

23. Hatano, M. (Chiba Univ.)

Redirected expression of Hox11 to developing thymocytes results in T cell development abnormality and malignancy.

24. Hattori, M. (Kyoto Univ.)

A new nuclear GTPase activating protein, SPA-1, possibly involved in the regulation of cell cycle.


Session IIIC T cell activation(Chairperson: Saito, T.)

25. Ohno, H. (Chiba Univ.)

Differential usage ofδand FcRγin the TCR complex by different T cell populations.

26. Inobe, M. (Hokkaido Univ.)

Identification of alternativery spliced form of the murine B7 having altered receptor binding and function properties.

27. Nariuchi, H. (Tokyo Univ.)

Humoral costimulators for Th cell activation.

28. Yoshikai, Y. (Nagoya Univ.)

Roles of viral superantigens in pathogenesis of viral infection.

Related posters

43. Zeng, Y-X. (TMIG, Tokyo)

Analysis of genes for signal transduction in T cell clones derived from young and old mice.

44. Kim, Y. H. (Kyungpook Univ., Korea)

The early events involved in T cell activation; Expression of murine G1 cyclins (CYL2 and CYL3) in T lymphocytes and aging.

45. Chien, Y. K. (Beijing Med. Univ.)

Effect of Lycium Barbarum L. on T lymphocyte activation.

46. Han Z-N. (Beijing Med. Univ.)

Study of phenotypic differentiation induced by PMA and PHA on CD3 and CD25 on human fetal thymocytes during early activation.

47. Zhou, S. (Inst. Basic Med. Sci., Beijing)

Purging of leukemic bone marrow by interleukin-2 lymphokine activated killer cells.

Session IV A Apoptosis(Chairperson: Tsubata, T.)

29. Iwata, M. (MKILS, Machida)

Involvement of Ca++-independent protein kinase C in glucocorticoid-induced apoptosis in thymocytes.

30. Hiramine, C. (Kagawa Med. School)

Negative selection and apoptosis in thymocytes induced by murine nursing thymic epithelial cell line.

31. Yonehara, S. (Pharm. Basic Res. Lab., JT Inc., Yokohama)

Involvement of apoptosis antigen Fas in negative selection/clonal deletion of human thymocytes induced by superantigen.

32. Tsubata, T. (Kyoto Univ.)

B cell apoptosis induced by antigen receptor crosslinking is blocked by T cell signal through CD40.


Session IV B Molecular mechanism of tolerance(Chairperson: Suzuki, G.)

33. Aiba, Y. (Kyoto Univ.)

Requirement of a second signal from antigen presenting cells in the clonal deletion of immature T cells.

34. Kubo, S. (Tokyo Univ.)

A novel form of selftolerance as studied by transgenic mice with autoreactive T cell receptorα andβchain genes: An unique positive selection in the thymus.

35. Suzuki, G. (NIRS, Chiba)

Co-stimulation in anergized T cells.


Session IV C In vivo mechanism of tolerance(Chairperson: Sasazuki, T.)

36. Sakaguchi, S. (RIKEN, Tsukuba)

A T cell-dependent mechanism of immunologic selftolerance: elimination of peripheral T cells expressing IL-2 receptors induces autoimmune disease in normal mice.

37. Toki, J. (Kansai Med. Univ., Osaka)

Analysis of induction and breakdown of tolerance by grafting cultured thymuses into SCID mice.

38. Fukui, Y. (Kyushu Univ.)

Positive selection of T-cell repertoire requires interaction with major histocompatibility complex on hematopoietic cells.


Session I Mechanisms of lymphocyte growth and differentiation(Chairperson: Hirokawa, K.)

S1. Nordin, A. A. (NIH, USA)

Transcriptional expression of cyclin-dependent kinases and cyclins during the activation of murine T lymphocytes.

S2. Tsukada, S. (Howard Hughes Med. Inst., UCLA)

Involvement of Brutons tyrosine kinase (Btk) in the pathogenesis of human and mouse immunodeficiency syndromes.

S3. Nishikawa, S. (Kyoto Univ.)

How B precursor cells are driven to cycle?


Session II Hematopoietic stem cells and T cell development(Chairperson: Minato, N.)

S4. Shortman, K. (The Walter and Eliza Hall Inst.)

Parallel development of dendritic cells and T cells within the thymus.

S5. Van Ewijk, W. (Erasmus Univ., The Netherlands)

ER-MP12 antigen, a new cell surface marker on mouse bone marrow cells with thymus-repopulating ability.

S6. Weissman, I. L. (Stanford Univ.)

Hematopoietic stem cell and thymic cell maturation.