It is our great pleasure to host the 7th KTCC meeting, which is the 2nd international conference. We expect that the most updated findings on the cellular and molecular mechanisms of hematopoiesis, T cell development and related fields will be presented and discussed at this meeting. This meeting will be attended by many of the leading members in these fields from all over the world. We hope that this meeting will be a stepping stone not only to the intensive investigation of the mechanisms of T cell development, but also to the unfolding of our work into a leading field in biology or natural science.

Organizers:

Yoshimoto KATSURA
Katsuiku HIROKAWA
Hiroshi HIAI
Nagahiro MINATO

Date: October 2−4, 1997

Site: Kyodai−Kaikan

Symposium 1 Origin and commitment of the T cell progenitor

（Chairpersons: K. Shortman and Y. Katsura）

S1. H. Nakauchi (Tsukuba Univ.)

The hematopoietic stem cell and lymphoid commitment.

S2. E. Dzierzak (Erasmus Univ., The Netherlands)

Embryonic origins of hemato/lymphoid cells.

S3. K. Akashi (Stanford Univ., USA)

Search for common lymphoid progenitor.

S4. H. Kawamoto (Kyoto Univ.)

Commitment of the hematopoietic stem cell to T, B and myeloid lineages at the earliest stage of hematopoiesis in the fetal liver.

S5. K. Shortman (Hall Inst., Australia)

T cells or dendritic cells as a developmental option: Control by cytokines and transcription factors.

Workshop 1 T cell progenitors

Chairpersons: A. Globerson and S. Habu

1. N. Takakura (Kumamoto Univ. Sch. Med.)

Expression and characterization of endothelial cell receptors in hematopoietic cells.

2. K. Hozumi (Tokai Univ. Sch. Med.)

The germ-line transcript of the TCRα gene is regulated by the promoter located within the Jα49 locus in immature T cells.

3. A. Muraguchi (Toyama Med. Pharm. Univ.)

Characterization and transcriptional regulation of human RAG genes.

4. H. Ishikawa (Keio Univ. School Med.)

T cell lineage-committed precursors colonize in multiple tiny clusters in the intestinal mucosa.

5. A. Globerson (Weizmann Inst. Sci,. Israel)

Alternative pathways of T cell development in the aging thymus.

Workshop 2 (Part I) Molecular mechanisms of lymphocyte differentiation（Chairperson: K. Ikuta）

6. K. Maki (Kyoto Univ.)

Introduction of Bcl-2 transgenes rescuesαβbut not γδ T-cell development in IL-7 receptor-deficient mice.

7. S. K. Ye (Kyoto Univ.)

Transgenic γδ T-cell development in IL-7 receptor-deficient mice.

8. H. Arase (Chiba Univ. Sch. Med.)

Regulation of Th1/Th2 response by NK1.1+ T cells and CD8+CD44+ T cells.

9. T. Fukuda (Chiba Univ. Sch. Med.)

Disruption of the Bc16 gene results in an impaired germinal center formation.

Panel disussion Thymic microenvironment（Chairpersons: W. van Ewijk and K. Hirokawa）

Panelists

Pn1. K. Hirokawa (Tokyo Medical & Dental Univ.)

Hypothalamic control of thymic function.

Pn2. H. Hiai (Kyoto Univ.)

NK1+ T lymphomas spontaneously developing in thymectomized (SL/Kh x AKR/Ms) F1 mice.

Pn3. W. van Ewijk (Erasmus Univ., The Netherlands)

Crosstalk in the mouse thymus is differentially regulated by subsets of developing T cells.

Pn4. R. Boyd (Monash Medical School, Australia)

Ontogenesis of the thymic microenvironment -- Is there a primordial epithelium precursor?

Discussants

10. K-U. Hartmann, B. Engelhardt (Philipps Univ., Germany)

Expression of different cell adhesion molecules in lymphoid cell aggregates during the fetal development of mice.

11. G. Anderson and E. J. Jenkinson (Univ. Birmingham, UK)

Outcomes and overlap of positive and negative selection in the thymus.

12. H. Wang, D. H. Dong, X. W. Pang, J. M. Feng and W. F. Chen (Beijing Med. Univ., China)

A thymic stromal cell and thymocyte shared molecule PF18-3 is involved in thymocyte apoptosis.

13. M. Itoi and T. Amagai (Meiji Univ. of Ori. Med.)

The proliferation and differentiation of thymic epithelial cells of mouse embryo.

14. H. Yamamoto (Osaka Univ.)

Cellular analysis of transgenic mice expressing a novel thymic epithelial cell antigen.

15. M. Kasai (Natl. Inst. Infect, Dis.)

Distribution and function of H2-DM in cortical and medullary thymic epithelial cells

16. W. T. V. Germeraad, Y. ﾐF. Jiang, J. de Kruif, P. Naquet, W-F. Chen, T. Logtenberg and

W. van Ewijk (Erasmus University Rotterdam, Univ. Hospital Utrecht, The Netherlands, Center dﾕImmunol. deMarseille-Luminy, France, Beijing Med. Univ., China）

Subtractive isolation of phage-displayed single chain antibodies to thymic stromal cells.

17. B. J. Classon, L.Barnett, R. Li, R. L. Boyd and F. Koontgen (Hall Inst., Monash Med. Sch.,　Australia)

Targeted disruption of the gene encoding the lymphostromal cell membrane molecule TSA-1.

Symposium 2 Transcription factors involved in hematopoiesis and T cell development

（Chairpersons: H. Clevers and M. Yamamoto）

S6. H. Clevers (Academic Hospital Utrecht, The Netherlands)

TCFs control development in flies, frogs, colon and lymphocytes.

S7. M. Yamamoto (Tsukuba Univ.)

Regulation of the regulators: Upstream of the erythroid transcription factor GATA-1.

S8. M. Satake (Tohoku Univ.)

Expression and function of the AML1 gene in T cell receptor-mediated apoptosis of the T hybridoma line.

S9. E. V. Rothenberg (California Inst. Technol., USA)

Expression of transcription factors and their target genes during T-lineage commitment events.

S10. J. D. Engel (Northwestern Univ., USA)

Transgenic YAC rescue of the embryonic lethal phenotypes conferred by GATA-2 and GATA-3 germ line mutations.

S11. K. Georgopoulos (Cutaneous Biol. Res. Center, USA)

The Ikaros gene family in T lymphocyte development and homeostasis.

Workshop 2 Part II Molecular mechanisms of lymphocyte differentiation

（Chairpersons: J. D. Engel and N. Minato）

18. T. Sato (Tokai Univ. Sch. Med.)

Evidence that CD45 normally inhibits mitogenic and survival signaling in developing thymocytes.

19. M. Iwashima (Mitsubishi Chemical Corporation)

Lck functions as a kinase and an adapter molecule in the TCR signaling pathway but is not required for superantigen stimulation.

20. A. Kosugi (Osaka Univ.)

Localization of CD3ζchains of detergent-insoluble glycosphingolipid -enriched complexes (DIG) of the plasma membrane.

21. N. Tosa (Hokkaido Univ.)

Characterizaiton of DIG1 molecule, a G-protein coupled receptor in thymus.

22. M. Yamashita (Res. Inst. Biol. Sci., Science Univ. of Tokyo)

Requirement for p56lck tyrosine kinase activation in T helper subset differentiation.

23. G. Suzuki (Natl. Inst. Radiol. Sci.)

A role of CTLA-4 co-stimulation on Th differentiation.

24. S. Adachi (Mitsubishi Kasei Inst. Life Sci.)

Regulation of thymocytes lineage commitment by the intracellular Ca2+ level.

25. Y. Tokoro (Tsukuba Univ.)

Preparation of monoclonal antibodies specific for recombinant fusion protein containing extracellular region of mouse pTα chain.

26. S. Fujimoto (Kyoto Univ.)

A newly cloned gene expressed in the early stages of T cell development.

27. D. Kioussis (NIMR, UK)

Locus control region function and heterochromatin-induced position effect variegation.

Workshop 3 Part I T cell selection and maturation （Chairperson: K. Onoe）

28. H. Suzuki (Keio Univ. Sch. Med.)

Extent of TCR aggregation is crucial in determining positive and negative selection of immature thymocyte.

29. N. Watanabe (Chiba Univ. Sch. Med.)

The quantity of TCR signaling determines positive selection and lineage commitment.

30. M. Homma (Teikyo Univ. Sch. Med.)

Defective de novo thymocyte maturation in cyclosporin A induced autoimmunity: Expression of costimulatory and activation molecules.

31. C. Iwabuchi (Hokkaido Univ.)

Positive and negative selection of NK-T cells in transgenic mice bearing MHC classII restricted TCR

Symposium 3 Molecular mechanism of T cell differentiation（Chairpersons: A. Singer and T. Saito）

S12. A. M. Kruisbeek (The Netherlands Cancer Inst., The Netherlands)

Genetic evidence for a role for the CD3-γchain in T cell development.

S13. Y. Tanaka (Centre dﾕimmunologie, Marseille, France)

γ-radiation rescues TCRα gene rearrangement in CD3ε-deficient mice.

S14. T. Saito (Chiba Univ.)

A new component of pre/pro-TCR complex on immature thymocytes.

S15. Y. Takahama (Tsukuba Univ.)

Molecular singnals that induce positive selection of thymocytes.

S16. A. Singer (NCI, NCH, USA)

Lineage commitment and positive selection of developing thymocytes.

Workshop 3 Part II T cell selection and maturation （Chairpersons: D. Kioussis and G. Suzuki）

32. H. Kishi (Toyama Med. Pharm. Univ.)

Requirement of lactate dehydrogenase-A for the cell-surface expression of immature thymocyte antigen-1.

33. T. Takahashi (Tokyo Metro. Inst. Cerontol.)

A mechanism of thymic/T cell-control of self-reactive T cells.

34. E. Nakamura (Kyoto Univ.)

Involvement of NK1+DNαβT cells and endogeneous IL-4 in non-MHC restricted rejection of embryonal carcinoma in genetically resistant mice.

35. A. Hasegawa (Science Univ. of Tokyo)

Regulation of T cell autoreactivity to MHC class II by controlling CD80 (B7-1) expression on B cells.

36. D. Kioussis (NIMR, UK)

Altered peptide ligands (APL) and positive/negative selection signals.

37. S. Mandard (Inst. Biol. Pasteur., France)

Nuclear expression of a Cb1-immunoreactive protein CAPA 80 during thymic apoptosis.

38. F. Tian (The 4th Military Med. Univ., China)

The cloning of a novel primates T cell activation antigen.

39. N. Sakaguchi (Tokyo Metro. Inst. Gerontol.)

A spontaneous rheumatoid arthritis-like disease in mice: Thymic/T cell dependency of the disease.

40. K. Imanishi (Tokyo Womenﾕs Medical College)

Extrathymic maturation of migrating human thymic single positive T cells.