Welcome to the KTCC meeting.

It is our great pleasure to host The 3rd International Workshop of KTCC meeting. This meeting will be attended by many of the leading members in the field of T cell research from all over the world. We expect that the most up to date findings on the cellular and molecular mechanisms of the development and functions of T cells and related fields will be presented and discussed at this meeting. We hope that this meeting will be a stepping stone not only to the intensive investigation of T cells, but also to the unfolding of our work in a leading field I biology and medical science.

Organizing committee:

Yoshimoto KATSURA (Chairperson)
Nagahiro MINATO
Hiromitsu NAKAUCHI
Takashi SAITO

Program of the 12rd KTCC meeting

Date: April 3−5, 2002

Site: Heian−Kaikan


Session I Thymic environment(Chairpersons: Owen, J. J. T. and Van Ewijk, W.)

1. Van Ewijk, W. (Erasmus University, Netherlands)

Rapid conversion of thymic microenvironments in vitro.

2. Jenkinson, E. J. (Medical school University of Birmingham, UK)

Entry into the thymic microenvironment triggers Notch activation in migrant T-cell prognitors.


3. Hollaender, G. A.(University of Basel, Switzerland)

Wnts regulatethe expressionof FoxN1, the gene defective in thymic epithelial cells of nude mice.

4. Staal, F. J. T. (Erasmus University, Netherlands)

Wnt signaling and the TCF-1 transcription factor in thymocyte differentiation.

5. Sano, S. (Dept. of Dermatology, Osaka University)

The role of Stat3 in thymic epithelial cells in the maintenance of thymic architecture and thymocyte survival.

6. Itoi, M. (Meiji University of Oriental Medicine)

Defects in thymus development in Patch (Ph) mutant mice.

7. Boyd, R.   (Monash University Medical School, Australia)

Rejuvenation of thymic structure and function through inhibition of steroids.


8. Kasai, M. (National Institute of Infectious Diseases)

Study on the molecular mechanism of MHC class II restricted antigen-presenting process in thymic epithelial cell lines.

9. Utsuyama, M. (Tokyo Medical and Dental University)

Glia maturation factor produced by thymic epithelial cells plays a role in T cell differentiation in the thymic microenvironment.

10. Chidgey, A. (Monash University Medical School, Australia)

Differential gene expression patterns between thymic cortical and medullary epithelium.


Session 2 T cell progenitors(Chairpersons: Koyasu, S. and Kincade, P. W.)

1. Kawamoto, H. (Kyoto University)

Prethymic and intrathymic pathways of the commitment of multipotent progenitors toward T cell lineage.

2. Petrie, H. T. (Memorial Sloan-Kettering Cancer Center, USA)

Control of lineage commitment and lymphopoietic development by precursor migration within the steady-state thymus.


3. Kincade, P. W. (Oklahoma Medical Research Foundation, USA)

The earliest lymphocyte precursors in adult bone marrow.

4. Wu, L. (The Walter and Eliza Hall Institute of Medical Research, Australia)

Characterization of thymus seeding precursor cells from mouse bone marrow.

5. Yoshida, H. (RIKEN Center for Allergy and Immunology)

Lineage negative IL-7Rαpositiveα4β7-integrin positive cells in fetal liver are the population committed to T-lineage lymphocyte and /or peripheral lymphoid organ inducer cells.


6. Kondo, S. (Toyama Medical Pharm. University)

Involvemen of glial cell line derived neurotrophic factor receptor-α1 in T cell development in thymus.

7. Ikawa, T. (Kyoto University)

T cell progenitors preferentially circulate in murine early embryos.

8. Lu, M. (Kyoto University)

The hierarchy in the developmental potential among early T cell progenitors in the adult thymus revealed by a clonal assay.


Session 3 T cell differentiation (I)(Chairpersons: Habu, S. and Malissen, B)

1. Singer, A. (Experimental Immunology Branch, NCI, USA)

Kinetic signaling as the basis of the CD4/CD8 lineage decision in the thymus.

2. Owen, J. J. T. (Oklahoma Medical Research Foundation, USA)

Live cell imaging of immunological synapse formation during thymocyte selection.


3. Anderson, G. (Medical school University of Birmingham, UK)

Studies on thymocyte-epithelial cell interactions in positive selection induction.


4. Kobayashi, Y. (Nagoya City University)

Molecules involved in apoptosis in the human thymus.

5. Uenishi, H. (National Institute of Agrobiological Sciences)

Complete genomic sequence around joining segments and constant regions of swine T cell receptor α/δlocus reveals striking resemblance of this region in mammals.

6. Wei, X. -C.   (Toyama Med. Pharm. University)

Characterization of chromatin structure and enhancer elements for murine Recombination activating gene-2.

7. Przybylski, G. K. (Institute of Human Genetics, Polish Academy of Science, Poland)

Identification of a new cluster of T-cell receptorδrecombining elements.


Session 4 T cell differentiation (II)(Chairpersons: Nakauchi, H. and MacDonald, H. R.)

1. MacDonald, H. R. (University of Lausanne, Switzerland)

Distinct functions of Notch 1 in T cell development revealed by tissue and stage specific gene targeting.

2. Zamoyska, R. (NIMR, UK)

The Role of scr-family kinases in T cell survival and homeostatic proliferation.


3. Udaka, K.     (Kyoto University )

Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage.

4. Nakayama, T. (Chiba University)

Regulation of Th2 cell differentiation b polycomb group gene products.

5. Oteki, T. (Keio University School of Medicine)

Distinct sets of IL-15 producing cells are required for development of memory CD8 T, NKT and intestinal intraepithelial T cells.

6. Ichii, H. (Chiba University)

Role of Bc16 in maintenance of memory CD8+ T cells.

7. Regnier, D. L. (Institute de Biologie de Lille, France)

The proto-oncogene c-cbl codes for an alternative spliced nuclear isoform associated with the occurrence of thymic apoptosis.


8. Itoh, Y. (Shiga University of Medicinal Science)

The role of antigen presenting cells and partial agonists in Th1 and Th2 differentiation.

9. Kikuchi, K. (Hokkaido University)

TNF-αbut not LPS enhances preference of murine DC for Th2 differentiation.

10. Yamashita, M. (PRESTO)

Regulation of CATA3-dependent histone hyperacetylation at the IL-4 and IL-13 gene loci by the Ras-ERK MAPK cascade.

11. Suzuki, H. (Yamaguchi University School of Medicine)

Critical roles of the GTPase Rac1 in positive selection of thymocytes.


Session 5 T cell trafficking(Chairpersons: Takahama, Y. and Singer, A.)

1. Fukui, Y. (Kyushu University)

A novel cytoskeleton regulating protein DOCK2: its critical role in migration and function of lymphocytes.

2. Takahama, Y. (RIKEN&Univ. Tokushima)

Role for chemokines in T cell emigration from the thymus.


3. Nagasawa, T. (Research Institute Osaka Medical Center for Maternal and Child Health)

The earliest stages of B cell development require a chemokine SDF-1/PBSF.

4. Schmidt, C. A. (Deutsches Rheuma-Forschungszentrum Berlin, Germany)

Two subsets of peripheral blood naive T helper cells with distinct T-cell receptor excision circles content indicate peripheral homeostatic expansion of recent thymic emigrants in adult humans .


5. Ueno, T. (RIKEN&Univ. Tokushima)

CCL19 expression in the newborn thymus.


Session 6 T cell activaion and Signaling (I)(Chairpersons: Saito, T. and Malissen, M.)

1. Saito, T. (Chiba University)

Regulation of immune synapse formation through anchoring lipid raft to cytoskeleton.

2. Malissen, B. (INSERM-CNRS-Univ. Med., France)

Spontaneous exaggerated T helper type 2 immunity in mice with a point mutation in LAT.


3. Kosugi, A. (Osaka University)

Regulation of tyrosine phosphorylation in lipid raft-mediated T cell activation.

4. Tanaka, Y. (Kyoto University)

Recognition mechanism of nonpeptide antigens by human γδT cells.

5. Aiba, Y. (Tokyo Medical and Dental University)

Role of CD40 signaling in selection and maturation of B cells in vivo.


6. Kuwahara, K. (Kumamoto University School of Medecine)

Germinal center-associated DNA-primase GANP is unregulated in antigen-driven B cells stimulated with T cell-dependent antigen in mice.

7. Nishimura, M. (National Institute of Radioligical Sciences)

Selection of thymocytes hyper-responsive to interleukins 7 and 9 at preleukemic stage in split-dose irradiated B6C3F1 mice.

8. Shibuya, K. (RIKEN)

Successful gene transfer into human CD4 positive na夫e T cells mediated by lentiviral vector.

9. Tomita, S. (RIKEN)

Disruption of the aryl hydrocarbon receptor nuclear translocator (Arnt) gene in T cells results in resistance to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-induced thymic involution.

10. Kina, T. (Kyoto University)

Immune disorders in CD45.1 congenic mice of BALB/c background.


Session7 T cell activation and Signaling (II)(Chairpersons: Minato, N. and Zamoyska, R.)

1. Minato, N. (Kyoto University)

Role of small molecular-mass GTPase Rap1 in the regulation of T cell activation and control of hematopoiesis.

2. Koretzky, G. (University of Pennsylvania and Investigator and Director, USA)

Adaptor molecules in T-cell signal transduction.


3. Ikuta, K. (Kyoto University)

The IL-7 receptor controls the accessibility of the TCRγlocus by Stat5 and Histone acetylation.

4. Nakase, H. (University of Tokushima)

Methylation of recombination signal sequence affects RAG1/2 activities.

5. Germeraad, W. T. V. (Crucell, Netherlands)

A human monoclonal antibody fused to the tumor antigen MAGE-1 targeted to dendritic cells results in CTL and Th responses in vitro.


6. Takahashi, S. (Chiba University)

In vivo requirement for inhibitory signals through CTLA-4.

7. Sakakibara, M. (Chiba University)

Negative regulation of immune synapse formation by anchoring lipid raft to v cytoskeleton through Cbp-EBP50-ERM assembly.

8. Otsuka, M. (Chiba University)

Identification of a novel ITAM-containing cell surface molecule inducing T cell Activation.

9. Kubagawa, H. (University of Alabama, USA)

Paired immunoglobulin-like receptors.


Session 8 Poster discussion(Chairpersons: Nakayama, T., Nagasawa, T. and Holl穫der, G. A.)


Session 9 Peripheral tolerance(Chairpersons: Sakaguchi, S. and Coutinho, A.)

1. Coutinho, A. (Institute Gulbenkian de Ciencia, Portugal)

A model for developmentally acquired natural self-tolerance.

2. Sakaguchi, S. (Kyoto University)

Immunologic self-tolerance maintained by CD25+CD4+ regulatory T cells.


3. Takahashi, T. (Kyoto University)

Thymic generation of CD25+CD4+ regulatory T cells: their possible high self- reactivity.

4. Hori, S. (Institute Gulbenkian de Ciencia, Portugal)

Specificity requirements for effector functions and selection of CD25+CD4+ regulatory T cells in anti-myelin basic protein T cell receptor transgenic mice.


Session 10 Transcription factors(Chairpersons: Satake, M. and Murre, C.)

1. Murre, C. (University of California, USA)

The activity of the helix-loop-helix protein E47 is modulated to promote thymocytes commitment, cellular expansion and developmental progression.

2. Rothenberg, E. V. (California Institute of Technology, USA)

A transcription factor regulatory network for T-lineage specification.


3. Satake, M. (Tohoku University)

Role of AML-1 as a potent repressor for GATA-3 expression in helper T cell differentiation.

4. Sato, T. (Tokai University)

The expression of a translation initiation factor eIF4E is developmentally regulated in thymocytes and associated with functional competence.

5. Kakinuma, S. (National Institute of Radiological Sciences)

Carcinogen-dependent alteration of Ikaros in murine T-cell lymphomas.

6. Fujimoto, S. (Kyoto University)

Derailed development of thymic progenitors by overexpression of the helix-loop- helix inhibitor Id2